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sheep anti mouse adamts1  (Novus Biologicals)


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    Novus Biologicals sheep anti mouse adamts1
    Sheep Anti Mouse Adamts1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+anti+mouse+adamts1/Human%2FMouse+ADAMTS1+Antibody/pm34135477-60-6-10
    Average 94 stars, based on 13 article reviews
    sheep anti mouse adamts1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Variant Assay:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Solvent:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Residue:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    RNA Expression:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    RNA Sequencing:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Expressing:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Gene Expression:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Immunostaining:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Labeling:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Staining:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Immunolabeling:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Animal Model:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Protein-Protein interactions:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Membrane:

    Article Title: ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5- 22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat antirabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).

    Article Title: ADAMTS1 , MPDZ, MVD , and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Article Snippet: Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.Confocal images were acquired from a Nikon spinning disk W1 confocal microscope and images were processed using ImageJ software.. The following primary antibodies were used: sheep anti-mouse ADAMTS1 (AF5867-SP; Novus Biologicals, Centennial, CO, USA), rabbit anti-mouse MPDZ (42-2700; Thermofisher Scientific, Waltham, MA, USA), rabbit anti-mouse MVD (PA5-22164; Thermofisher Scientific), and sheep anti-mouse SEZ6 (PA5-47683; Thermofisher Scientific).. The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).The following secondary antibodies were used: Donkey anti-sheep 564 nm for ADAMTS1 and SEZ6 (Invitrogen), Goat anti-rabbit 594 nm for MPDZ (Invitrogen), and Goat anti-rabbit 488 nm for MVD (Invitrogen).



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    In silico analysis of ADAMTS1 and endothelial markers in glioma samples from Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) glioblastoma multiforme- Low Grade Gliomas (GBM-LGG) projects. ( A ) Graph representing ADAMTS1 gene expression among different grades of glioma according to WHO classification, analyzing both CGGA and TCGA GBM-LGG datasets. TCGA data are analyzed with cBioPortal platform (****, p < 0.0001; *, p < 0.05 in unpaired t -test, using WHO II patients as control. Median and interquartile range are indicated for each group); ( B ) Scatter plots representing correlation analyses between gene expression levels of ADAMTS1 and endothelial-related genes CDH5, EPHA2, FLT1 and KDR ( R = Pearson correlation coefficient); ( C ) Kaplan–Meier survival curves for low and high gene expression levels of ADAMTS1 considering all glioma grades, in both CGGA and TCGA GBM-LGG datasets.

    Journal: Biomolecules

    Article Title: ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression

    doi: 10.3390/biom11010044

    Figure Lengend Snippet: In silico analysis of ADAMTS1 and endothelial markers in glioma samples from Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) glioblastoma multiforme- Low Grade Gliomas (GBM-LGG) projects. ( A ) Graph representing ADAMTS1 gene expression among different grades of glioma according to WHO classification, analyzing both CGGA and TCGA GBM-LGG datasets. TCGA data are analyzed with cBioPortal platform (****, p < 0.0001; *, p < 0.05 in unpaired t -test, using WHO II patients as control. Median and interquartile range are indicated for each group); ( B ) Scatter plots representing correlation analyses between gene expression levels of ADAMTS1 and endothelial-related genes CDH5, EPHA2, FLT1 and KDR ( R = Pearson correlation coefficient); ( C ) Kaplan–Meier survival curves for low and high gene expression levels of ADAMTS1 considering all glioma grades, in both CGGA and TCGA GBM-LGG datasets.

    Article Snippet: Then, they were blocked with 5% low-fat milk and incubated with a sheep anti-human ADAMTS1 antibody (AF5867, R&D Systems, Minneapolis, MN, USA).

    Techniques: In Silico, Gene Expression, Control

    Evaluation of ADAMTS1 gene expression and plasticity in GICs and GBM cell lines. ( A ) Graphs representing mRNA fold change expression of ADAMTS1 , CDH5 and ENG in independent GICs as spheres and undergoing differentiation (standard deviation is not included in these graphs as they represent individual tumor samples); ( B ) Graph representing mRNA fold change expression of stemness markers PROM1 and SOX2 in monolayer cultures and tumor-spheres derived from U87-MG and U251-MG cells ( n ≥ 3 samples for all cell lines); ( C ) Graphs representing mRNA fold change expression of ADAMTS1 in monolayer cultures, tumor-spheres, and differentiation status, derived from U87-MG, and U251-MG cells ( n ≥ 3 samples for all cell lines). **, p < 0.001; *, p < 0.01; and *, p < 0.05.

    Journal: Biomolecules

    Article Title: ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression

    doi: 10.3390/biom11010044

    Figure Lengend Snippet: Evaluation of ADAMTS1 gene expression and plasticity in GICs and GBM cell lines. ( A ) Graphs representing mRNA fold change expression of ADAMTS1 , CDH5 and ENG in independent GICs as spheres and undergoing differentiation (standard deviation is not included in these graphs as they represent individual tumor samples); ( B ) Graph representing mRNA fold change expression of stemness markers PROM1 and SOX2 in monolayer cultures and tumor-spheres derived from U87-MG and U251-MG cells ( n ≥ 3 samples for all cell lines); ( C ) Graphs representing mRNA fold change expression of ADAMTS1 in monolayer cultures, tumor-spheres, and differentiation status, derived from U87-MG, and U251-MG cells ( n ≥ 3 samples for all cell lines). **, p < 0.001; *, p < 0.01; and *, p < 0.05.

    Article Snippet: Then, they were blocked with 5% low-fat milk and incubated with a sheep anti-human ADAMTS1 antibody (AF5867, R&D Systems, Minneapolis, MN, USA).

    Techniques: Gene Expression, Expressing, Standard Deviation, Derivative Assay

    Effect of ADAMTS1 knockout in GBM cells. ( A ) Graph representing mRNA fold change expression of ADAMTS1 in U87-MG, U251-MG, and their respective ATS1ko clones ( n ≥ 3 for all cells) (**, p < 0.01; and *, p < 0.05. WT U87-MG and U251-MG cells were used as controls for statistical analyses, respectively); ( B ) Representative images of tumor-spheres derived from WT U87-MG and its ATS1ko clones, and graph representing their measures; ( C ) Representative images of tumor-spheres derived from WT U251-MG and its ATS1ko clones, and graph representing their measures; ( D ) Representative images (original and WimSprout processed) of 24 h sprouting tumor-spheres derived from WT U251-MG and U251-ATS1ko2 and ko3 clones; ( E ) graphs representing sprout number per sphere (top) and average length (bottom) according to WimSprout quantification of sprouting spheres as in ( E ) ( n ≥ 3 samples for all cells) (**, p < 0.01; and *, p < 0.05).

    Journal: Biomolecules

    Article Title: ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression

    doi: 10.3390/biom11010044

    Figure Lengend Snippet: Effect of ADAMTS1 knockout in GBM cells. ( A ) Graph representing mRNA fold change expression of ADAMTS1 in U87-MG, U251-MG, and their respective ATS1ko clones ( n ≥ 3 for all cells) (**, p < 0.01; and *, p < 0.05. WT U87-MG and U251-MG cells were used as controls for statistical analyses, respectively); ( B ) Representative images of tumor-spheres derived from WT U87-MG and its ATS1ko clones, and graph representing their measures; ( C ) Representative images of tumor-spheres derived from WT U251-MG and its ATS1ko clones, and graph representing their measures; ( D ) Representative images (original and WimSprout processed) of 24 h sprouting tumor-spheres derived from WT U251-MG and U251-ATS1ko2 and ko3 clones; ( E ) graphs representing sprout number per sphere (top) and average length (bottom) according to WimSprout quantification of sprouting spheres as in ( E ) ( n ≥ 3 samples for all cells) (**, p < 0.01; and *, p < 0.05).

    Article Snippet: Then, they were blocked with 5% low-fat milk and incubated with a sheep anti-human ADAMTS1 antibody (AF5867, R&D Systems, Minneapolis, MN, USA).

    Techniques: Knock-Out, Expressing, Clone Assay, Derivative Assay

    Analysis of ADAMTS1 in GBM cells and evaluation of their endothelial-like properties. ( A ) Graph representing mRNA fold change expression of ADAMTS1 in GBM cells: U87-MG, U251-MG, U373-MG and T98G (values are relative to U87-MG cell line) ( n ≥ 6 for all cell lines); ( B ) western blot analysis of ADAMTS1 in conditioned media of GBM cells. Black arrow points to full-length (FL) ADAMTS1. Red Ponceau staining was used as loading control; ( C ) graphs representing mRNA fold change expression of CDH5 , EPHA2 , FLT1 and KDR in GBM cells (values are relative to U87-MG cell line) ( n ≥ 3 for all genes except EPHA2 , n = 1); ( D ) representative images of 3D Matrigel-based assay of HUVEC (green square) and GBM cells, 24 h after seeding (white scale bar = 200 µm); EL+ (blue square) and EL− (red square) cells are indicated; ( E ) graph representing mRNA fold change expression of ADAMTS1 in GBM cells as monolayer (black bar) or in Matrigel assay (white bar) ( n ≥ 4 for all conditions). (****, p < 0.0001; ***, p < 0.001; **, p < 0.01, and *, p < 0.05).

    Journal: Biomolecules

    Article Title: ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression

    doi: 10.3390/biom11010044

    Figure Lengend Snippet: Analysis of ADAMTS1 in GBM cells and evaluation of their endothelial-like properties. ( A ) Graph representing mRNA fold change expression of ADAMTS1 in GBM cells: U87-MG, U251-MG, U373-MG and T98G (values are relative to U87-MG cell line) ( n ≥ 6 for all cell lines); ( B ) western blot analysis of ADAMTS1 in conditioned media of GBM cells. Black arrow points to full-length (FL) ADAMTS1. Red Ponceau staining was used as loading control; ( C ) graphs representing mRNA fold change expression of CDH5 , EPHA2 , FLT1 and KDR in GBM cells (values are relative to U87-MG cell line) ( n ≥ 3 for all genes except EPHA2 , n = 1); ( D ) representative images of 3D Matrigel-based assay of HUVEC (green square) and GBM cells, 24 h after seeding (white scale bar = 200 µm); EL+ (blue square) and EL− (red square) cells are indicated; ( E ) graph representing mRNA fold change expression of ADAMTS1 in GBM cells as monolayer (black bar) or in Matrigel assay (white bar) ( n ≥ 4 for all conditions). (****, p < 0.0001; ***, p < 0.001; **, p < 0.01, and *, p < 0.05).

    Article Snippet: Then, they were blocked with 5% low-fat milk and incubated with a sheep anti-human ADAMTS1 antibody (AF5867, R&D Systems, Minneapolis, MN, USA).

    Techniques: Expressing, Western Blot, Staining, Control, Matrigel Assay

    Effect of ADAMTS1 knockout in GBM cells co-cultured with HUVECs. Representative images of a co-culture Matrigel assay containing WT U251-MG (top row) or 3 U251-ATS1ko clones (second to fourth rows), co-cultured with HUVECs (second column, red). Third column is the resulting merge of U251 and HUVECs.

    Journal: Biomolecules

    Article Title: ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression

    doi: 10.3390/biom11010044

    Figure Lengend Snippet: Effect of ADAMTS1 knockout in GBM cells co-cultured with HUVECs. Representative images of a co-culture Matrigel assay containing WT U251-MG (top row) or 3 U251-ATS1ko clones (second to fourth rows), co-cultured with HUVECs (second column, red). Third column is the resulting merge of U251 and HUVECs.

    Article Snippet: Then, they were blocked with 5% low-fat milk and incubated with a sheep anti-human ADAMTS1 antibody (AF5867, R&D Systems, Minneapolis, MN, USA).

    Techniques: Knock-Out, Cell Culture, Co-Culture Assay, Matrigel Assay, Clone Assay

    Evaluation of GBM cells under hypoxia and effect of ADAMTS1 knockout. ( A – C ) Graphs representing mRNA fold change expression of ADAMTS1 ( A ), PROM1 ( B ), and ENG ( C ) in normoxic and hypoxic cultures of GBM cells: U87-MG, U251-MG, U373-MG and T98G; ( D ) graph representing mRNA fold change expression of PROM1 in hypoxic cultures of WT U87-MG and U87-ATS1ko clones; ( E ) graph representing mRNA fold change expression of PROM1 in hypoxic cultures of WT U251-MG and U251-ATS1ko clones. ( n ≥ 3 samples for all cell lines and conditions) (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; and *, p < 0.05).

    Journal: Biomolecules

    Article Title: ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression

    doi: 10.3390/biom11010044

    Figure Lengend Snippet: Evaluation of GBM cells under hypoxia and effect of ADAMTS1 knockout. ( A – C ) Graphs representing mRNA fold change expression of ADAMTS1 ( A ), PROM1 ( B ), and ENG ( C ) in normoxic and hypoxic cultures of GBM cells: U87-MG, U251-MG, U373-MG and T98G; ( D ) graph representing mRNA fold change expression of PROM1 in hypoxic cultures of WT U87-MG and U87-ATS1ko clones; ( E ) graph representing mRNA fold change expression of PROM1 in hypoxic cultures of WT U251-MG and U251-ATS1ko clones. ( n ≥ 3 samples for all cell lines and conditions) (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; and *, p < 0.05).

    Article Snippet: Then, they were blocked with 5% low-fat milk and incubated with a sheep anti-human ADAMTS1 antibody (AF5867, R&D Systems, Minneapolis, MN, USA).

    Techniques: Knock-Out, Expressing, Clone Assay

    Endothelial-like properties of melanoma cells and correlation with ADAMTS1 expression. ( a ) Representative images of 3D Matrigel-based assay of human melanoma cell lines, 24 h after seeding. 20,000 cells/well were cultured for MUM-2B, SK-MEL-147, C8161, MUM-2C and SK-MEL-103; and 30,000 cells/well for SK-MEL-28, G-361 and A-375 (white scale bar = 500 µm); ( b ) Graph representing mRNA fold change expression of ADAMTS1 in human melanoma cell lines. Values are relative to MUM-2B ( n = 21 for MUM-2B, n = 17 for MUM-2C, n = 11 for A-375, n = 5 for C8161 and SK-MEL-28, n = 6 for G-361, n = 15 for SK-MEL-103 and n = 9 for SK-MEL-147). EL+ and EL− phenotypes are indicated; ( c ) Graph representing mRNA fold change expression of ADAMTS1 in human melanoma cell lines, according to their EL+ or EL− phenotype (values are based in same data that C); ( d ) Heatmap showing differential gene expression between EL+ (including HUVECs) and EL− cell lines. Only significant differently expressed genes are depicted (47 upregulated and 420 downregulated, FDR < 0.05). Gene Expression Omnibus (GEO) ID samples are listed and color coded in ; ( e ) Representation of top ten GO Biological Processes after enrichment analysis using significantly upregulated genes in EL+ cells. Red line determined the limit of significance: -log (0.05). (****, p < 0.0001; ***, p < 0.001; and **, p < 0.01).

    Journal: Cancers

    Article Title: Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells

    doi: 10.3390/cancers12040801

    Figure Lengend Snippet: Endothelial-like properties of melanoma cells and correlation with ADAMTS1 expression. ( a ) Representative images of 3D Matrigel-based assay of human melanoma cell lines, 24 h after seeding. 20,000 cells/well were cultured for MUM-2B, SK-MEL-147, C8161, MUM-2C and SK-MEL-103; and 30,000 cells/well for SK-MEL-28, G-361 and A-375 (white scale bar = 500 µm); ( b ) Graph representing mRNA fold change expression of ADAMTS1 in human melanoma cell lines. Values are relative to MUM-2B ( n = 21 for MUM-2B, n = 17 for MUM-2C, n = 11 for A-375, n = 5 for C8161 and SK-MEL-28, n = 6 for G-361, n = 15 for SK-MEL-103 and n = 9 for SK-MEL-147). EL+ and EL− phenotypes are indicated; ( c ) Graph representing mRNA fold change expression of ADAMTS1 in human melanoma cell lines, according to their EL+ or EL− phenotype (values are based in same data that C); ( d ) Heatmap showing differential gene expression between EL+ (including HUVECs) and EL− cell lines. Only significant differently expressed genes are depicted (47 upregulated and 420 downregulated, FDR < 0.05). Gene Expression Omnibus (GEO) ID samples are listed and color coded in ; ( e ) Representation of top ten GO Biological Processes after enrichment analysis using significantly upregulated genes in EL+ cells. Red line determined the limit of significance: -log (0.05). (****, p < 0.0001; ***, p < 0.001; and **, p < 0.01).

    Article Snippet: Then, membranes were blocked with 5% low-fat milk and incubated with sheep anti-human ADAMTS1 (AF5867, R&D Systems, Minneapolis, MN, USA) and monoclonal mouse anti-Actin (sc-8432, Santa Cruz Biotechnology, Dallas, TX, USA) antibodies.

    Techniques: Expressing, Matrigel Assay, Cell Culture, Gene Expression

    ADAMTS1 inhibition affects in vitro endothelial-like phenotypic properties and endothelial-related signature. ( a ) Western blot analysis of conditioned media and cell lysates of ADAMTS1 in MUM-2B WT and ATS1-KO cells. Black arrows point full-length (FL) ADAMTS1. Red Ponceau staining and Actin were used as loading controls for conditioned media and cell lysates, respectively (uncut blots including a densitometry analysis are shown in ); ( b ) Representative images (original and WimTube filtered) of Matrigel assay for MUM-2B WT and ATS1-KO cells, 24 h after seeding 20,000 cells/well. Scatter plots represent the parameters resulting of WimTube analysis: total tubes, total branching points and total loops ( n = 12 for all groups, white scale bar = 500 µm); ( c ) Graphs representing mRNA fold change expression of CDH5 , ENG , EPHA2 , KDR , LAMC2 , TEK and TIE1 in MUM-2B WT and ATS1-KO cells ( n = 3–5 for WT, n = 3–6 for ATS1-KO1 and n = 2–4 for ATS1-KO2); ( d ) Graph representing mRNA fold change expression of CDH5 in C8161 WT and ATS1-KO cells ( n = 4 for all groups). (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; and *, p < 0.05. WT cells were used as control for statistical analyses).

    Journal: Cancers

    Article Title: Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells

    doi: 10.3390/cancers12040801

    Figure Lengend Snippet: ADAMTS1 inhibition affects in vitro endothelial-like phenotypic properties and endothelial-related signature. ( a ) Western blot analysis of conditioned media and cell lysates of ADAMTS1 in MUM-2B WT and ATS1-KO cells. Black arrows point full-length (FL) ADAMTS1. Red Ponceau staining and Actin were used as loading controls for conditioned media and cell lysates, respectively (uncut blots including a densitometry analysis are shown in ); ( b ) Representative images (original and WimTube filtered) of Matrigel assay for MUM-2B WT and ATS1-KO cells, 24 h after seeding 20,000 cells/well. Scatter plots represent the parameters resulting of WimTube analysis: total tubes, total branching points and total loops ( n = 12 for all groups, white scale bar = 500 µm); ( c ) Graphs representing mRNA fold change expression of CDH5 , ENG , EPHA2 , KDR , LAMC2 , TEK and TIE1 in MUM-2B WT and ATS1-KO cells ( n = 3–5 for WT, n = 3–6 for ATS1-KO1 and n = 2–4 for ATS1-KO2); ( d ) Graph representing mRNA fold change expression of CDH5 in C8161 WT and ATS1-KO cells ( n = 4 for all groups). (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; and *, p < 0.05. WT cells were used as control for statistical analyses).

    Article Snippet: Then, membranes were blocked with 5% low-fat milk and incubated with sheep anti-human ADAMTS1 (AF5867, R&D Systems, Minneapolis, MN, USA) and monoclonal mouse anti-Actin (sc-8432, Santa Cruz Biotechnology, Dallas, TX, USA) antibodies.

    Techniques: Inhibition, In Vitro, Western Blot, Staining, Matrigel Assay, Expressing, Control

    ADAMTS1 inhibition blocks tumorigenesis and alters tumor vasculature. ( a ) Table indicating the number of mice that developed tumors and the total number of injected mice of every experimental group (in parenthesis); ( b ) Graph representing final tumor weight of different experimental groups, according to panel A; ( c ) Representative pictures of tumors from SwN-Matrigel and NSG groups (black scale bar = 1 cm); ( d ) Graphs representing tumor evolution for each experimental group. ( e ) Representative images of EMCN immunofluorescence analysis of tumor sections from WT and ATS1-KO NSG xenografts (white scale bar = 100 µm); ( f ) Graphs representing tumor vasculature quantification of NSG xenografts: vessel density, vessel perimeter and percentage area ( n = 5 for WT; n = 7 for ATS1-KO1; n = 6 for ATS1-KO2). (***, p < 0.001 and **, p < 0.01. Tumors generated with WT cells were used as control for statistical analyses).

    Journal: Cancers

    Article Title: Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells

    doi: 10.3390/cancers12040801

    Figure Lengend Snippet: ADAMTS1 inhibition blocks tumorigenesis and alters tumor vasculature. ( a ) Table indicating the number of mice that developed tumors and the total number of injected mice of every experimental group (in parenthesis); ( b ) Graph representing final tumor weight of different experimental groups, according to panel A; ( c ) Representative pictures of tumors from SwN-Matrigel and NSG groups (black scale bar = 1 cm); ( d ) Graphs representing tumor evolution for each experimental group. ( e ) Representative images of EMCN immunofluorescence analysis of tumor sections from WT and ATS1-KO NSG xenografts (white scale bar = 100 µm); ( f ) Graphs representing tumor vasculature quantification of NSG xenografts: vessel density, vessel perimeter and percentage area ( n = 5 for WT; n = 7 for ATS1-KO1; n = 6 for ATS1-KO2). (***, p < 0.001 and **, p < 0.01. Tumors generated with WT cells were used as control for statistical analyses).

    Article Snippet: Then, membranes were blocked with 5% low-fat milk and incubated with sheep anti-human ADAMTS1 (AF5867, R&D Systems, Minneapolis, MN, USA) and monoclonal mouse anti-Actin (sc-8432, Santa Cruz Biotechnology, Dallas, TX, USA) antibodies.

    Techniques: Inhibition, Injection, Immunofluorescence, Generated, Control

    ADAMTS1 inhibition compromises the stemness capacities and endothelial-like phenotype in tumor xenografts. ( a–c ) Graphs representing mRNA fold change expression of NANOG , POU5F1 , PROM1 and SOX2 ( a ), ADAMTS1 ( b ) and CDH5 ( c ), in MUM-2B WT 2D cultured cells and NSG xenografts ( n = 4–6 for cells and n = 3–4 for NSG); ( d-e ) Graphs representing mRNA fold change expression of NANOG, POU5F1, PROM1 and SOX2 ( d ), and CDH5 ( e ) in NSG xenografts generated with WT and ATS1-KO cells ( n = 3 for WT, n = 5 for ATS1-KO1 and n = 4–6 for ATS1-KO2); ( f ) Representative images of IF analysis of WT and ATS1-KO NSG xenografts, at low and high magnification. For low magnification, columns from left to right: CDH5, EMCN, DAPI and merge; ( g ) Representative images of IHC staining of CDH5 and NANOG in consecutive sections of WT and ATS1-KO NSG xenografts; ( h ) Representative images of IHC co-staining of CDH5 and PAS, in WT and ATS1-KO NSG xenografts. (****, p < 0.0001; **, p < 0.01; and *, p < 0.05. White scale bar = 50 µm).

    Journal: Cancers

    Article Title: Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells

    doi: 10.3390/cancers12040801

    Figure Lengend Snippet: ADAMTS1 inhibition compromises the stemness capacities and endothelial-like phenotype in tumor xenografts. ( a–c ) Graphs representing mRNA fold change expression of NANOG , POU5F1 , PROM1 and SOX2 ( a ), ADAMTS1 ( b ) and CDH5 ( c ), in MUM-2B WT 2D cultured cells and NSG xenografts ( n = 4–6 for cells and n = 3–4 for NSG); ( d-e ) Graphs representing mRNA fold change expression of NANOG, POU5F1, PROM1 and SOX2 ( d ), and CDH5 ( e ) in NSG xenografts generated with WT and ATS1-KO cells ( n = 3 for WT, n = 5 for ATS1-KO1 and n = 4–6 for ATS1-KO2); ( f ) Representative images of IF analysis of WT and ATS1-KO NSG xenografts, at low and high magnification. For low magnification, columns from left to right: CDH5, EMCN, DAPI and merge; ( g ) Representative images of IHC staining of CDH5 and NANOG in consecutive sections of WT and ATS1-KO NSG xenografts; ( h ) Representative images of IHC co-staining of CDH5 and PAS, in WT and ATS1-KO NSG xenografts. (****, p < 0.0001; **, p < 0.01; and *, p < 0.05. White scale bar = 50 µm).

    Article Snippet: Then, membranes were blocked with 5% low-fat milk and incubated with sheep anti-human ADAMTS1 (AF5867, R&D Systems, Minneapolis, MN, USA) and monoclonal mouse anti-Actin (sc-8432, Santa Cruz Biotechnology, Dallas, TX, USA) antibodies.

    Techniques: Inhibition, Expressing, Cell Culture, Generated, Immunohistochemistry, Staining

    Inhibition of ADAMTS1 compromises melanoma sphere formation. ( a ) Graphs representing mRNA fold-change expression of NANOG, POU5F1, PROM1 , ADAMTS1 and CDH5 in MUM-2B WT 2D culture, primary and secondary melanoma spheres ( n = 4 for 2D culture, except for ADAMTS1 which is 12; n = 2–5 for primary spheres, and n = 4–6 for secondary spheres). 2D cultured cells were used as control for statistical analyses; ( b ) Violin plots representing volume of primary ( n = 49 for WT, n = 62 for ATS1-KO1, and n = 58 for ATS1-KO2) and secondary ( n = 46 for WT, n = 34 for ATS1-KO1 and n = 21 for ATS1-KO2) melanoma spheres. WT spheres volume was used as control for statistical analyses; ( c ) Representative images of WT and ATS1-KO primary melanoma spheres grown in CSC medium; ( d ) Graphs representing mRNA fold-change expression of NANOG and CDH5 in 2D culture ( n = 4–6 for ATS1-KO1 and n = 2–4 for ATS1-KO2) and primary melanoma spheres ( n = 2 for ATS1-KO1 and n = 4–7 for ATS1-KO2). 2D cultured conditions were used as control for statistical analyses; ( e ) Schematic protocol for melanoma spheres formation, using CSC medium and ADAMTS1+ CM; ( f ) Violin plots representing volume of WT and ATS1-KO primary melanoma spheres grown in CSC medium ( n = 49 for WT, n = 62 for ATS1-KO1, and n = 58 for ATS1-KO2) or in ADAMTS1+ CM ( n = 44 for WT, n = 48 for ATS1-KO1, and n = 49 for ATS1-KO2); ( g ) Representative images of WT and ATS1-KO primary melanoma spheres in ADAMTS1+ CM; ( h ) Violin plots representing WT and ATS1-KO1 primary melanoma spheres, grown in control CSC ( n = 27 for WT, and n = 44 for ATS1-KO1) or rhATS1+ CSC medium ( n = 35 for WT, and n = 28 for ATS1-KO1); ( i ) Representative images of WT and ATS1-KO1 primary melanoma spheres grown in control and rhATS1+ CSC medium. (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05. Violin plots indicate the median of every experimental group. White scale bar = 100 µm).

    Journal: Cancers

    Article Title: Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells

    doi: 10.3390/cancers12040801

    Figure Lengend Snippet: Inhibition of ADAMTS1 compromises melanoma sphere formation. ( a ) Graphs representing mRNA fold-change expression of NANOG, POU5F1, PROM1 , ADAMTS1 and CDH5 in MUM-2B WT 2D culture, primary and secondary melanoma spheres ( n = 4 for 2D culture, except for ADAMTS1 which is 12; n = 2–5 for primary spheres, and n = 4–6 for secondary spheres). 2D cultured cells were used as control for statistical analyses; ( b ) Violin plots representing volume of primary ( n = 49 for WT, n = 62 for ATS1-KO1, and n = 58 for ATS1-KO2) and secondary ( n = 46 for WT, n = 34 for ATS1-KO1 and n = 21 for ATS1-KO2) melanoma spheres. WT spheres volume was used as control for statistical analyses; ( c ) Representative images of WT and ATS1-KO primary melanoma spheres grown in CSC medium; ( d ) Graphs representing mRNA fold-change expression of NANOG and CDH5 in 2D culture ( n = 4–6 for ATS1-KO1 and n = 2–4 for ATS1-KO2) and primary melanoma spheres ( n = 2 for ATS1-KO1 and n = 4–7 for ATS1-KO2). 2D cultured conditions were used as control for statistical analyses; ( e ) Schematic protocol for melanoma spheres formation, using CSC medium and ADAMTS1+ CM; ( f ) Violin plots representing volume of WT and ATS1-KO primary melanoma spheres grown in CSC medium ( n = 49 for WT, n = 62 for ATS1-KO1, and n = 58 for ATS1-KO2) or in ADAMTS1+ CM ( n = 44 for WT, n = 48 for ATS1-KO1, and n = 49 for ATS1-KO2); ( g ) Representative images of WT and ATS1-KO primary melanoma spheres in ADAMTS1+ CM; ( h ) Violin plots representing WT and ATS1-KO1 primary melanoma spheres, grown in control CSC ( n = 27 for WT, and n = 44 for ATS1-KO1) or rhATS1+ CSC medium ( n = 35 for WT, and n = 28 for ATS1-KO1); ( i ) Representative images of WT and ATS1-KO1 primary melanoma spheres grown in control and rhATS1+ CSC medium. (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05. Violin plots indicate the median of every experimental group. White scale bar = 100 µm).

    Article Snippet: Then, membranes were blocked with 5% low-fat milk and incubated with sheep anti-human ADAMTS1 (AF5867, R&D Systems, Minneapolis, MN, USA) and monoclonal mouse anti-Actin (sc-8432, Santa Cruz Biotechnology, Dallas, TX, USA) antibodies.

    Techniques: Inhibition, Expressing, Cell Culture, Control

    Identification of EL and ECM molecules as poor prognosis factors in TCGA Uveal Melanoma Project (TCGA-UVM). ( a ) Kaplan–Meier survival curves for low and high gene expression levels of EL markers CDH5 and KDR ; ( b ) Scatter plot representing Pearson correlation analysis between gene expression levels of CDH5 and endothelial-related KDR and TIE1 ; ( c ) Representation of top ten GO Biological Processes after enrichment analysis using genes that positively correlated (q-value < 0.05) with CDH5 . Red line determined the limit of significance: -log (0.05); ( d ) Box graph representing ADAMTS1 expression among different clinical stages of human uveal melanoma, from stage IIA to IV ( n = 4 for IIA, n = 32 for IIB, n = 27 for IIIA, n = 10 for IIIB, n = 3 for IIIC and n = 4 for IV); ( e ) Kaplan–Meier survival curves for low and high gene expression levels of extracellular proteases ADAMTS4, ADAMTS5, ADAMTS9, ADAMTS12, ADAMTS2 and ADAMTS14 ; ( f ) Scatter plot representing Pearson correlation analysis between gene expression levels of CDH5 and extracellular proteases ADAMTS4 , ADAMTS9 , ADAMTS12 and ADAMTS2 . (Survival probability is depicted in correlation analysis plots (panels B and F) with light and dark red dots, representing low and high survival probability, respectively. r = Pearson correlation coefficient).

    Journal: Cancers

    Article Title: Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells

    doi: 10.3390/cancers12040801

    Figure Lengend Snippet: Identification of EL and ECM molecules as poor prognosis factors in TCGA Uveal Melanoma Project (TCGA-UVM). ( a ) Kaplan–Meier survival curves for low and high gene expression levels of EL markers CDH5 and KDR ; ( b ) Scatter plot representing Pearson correlation analysis between gene expression levels of CDH5 and endothelial-related KDR and TIE1 ; ( c ) Representation of top ten GO Biological Processes after enrichment analysis using genes that positively correlated (q-value < 0.05) with CDH5 . Red line determined the limit of significance: -log (0.05); ( d ) Box graph representing ADAMTS1 expression among different clinical stages of human uveal melanoma, from stage IIA to IV ( n = 4 for IIA, n = 32 for IIB, n = 27 for IIIA, n = 10 for IIIB, n = 3 for IIIC and n = 4 for IV); ( e ) Kaplan–Meier survival curves for low and high gene expression levels of extracellular proteases ADAMTS4, ADAMTS5, ADAMTS9, ADAMTS12, ADAMTS2 and ADAMTS14 ; ( f ) Scatter plot representing Pearson correlation analysis between gene expression levels of CDH5 and extracellular proteases ADAMTS4 , ADAMTS9 , ADAMTS12 and ADAMTS2 . (Survival probability is depicted in correlation analysis plots (panels B and F) with light and dark red dots, representing low and high survival probability, respectively. r = Pearson correlation coefficient).

    Article Snippet: Then, membranes were blocked with 5% low-fat milk and incubated with sheep anti-human ADAMTS1 (AF5867, R&D Systems, Minneapolis, MN, USA) and monoclonal mouse anti-Actin (sc-8432, Santa Cruz Biotechnology, Dallas, TX, USA) antibodies.

    Techniques: Gene Expression, Expressing